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Get a deeper insight into our products. We have compiled collected a collection of frequently asked questions, data sheets, publications and PHABIOC media for you.
How to prepare a side-by-side diffusion cell – using PermeaPad® Barrier
How to prepare a franz Cell – using PermeaPad® Barrier
How to use PermeaPad GIT Plate – PHABIOC
The permeation coefficients are in the same order of magnitude as for PAMPA.
You can split the barrier and dissolve/ extract with methanol or ethanol.
After your investigation you can dissolve/extract the active with ethanol or methanol by splitting the barrier to calculate the amount of active that remains in the membrane.
At the following link, you can download a review (free access) describing integrity testing. It is described in chapter 4.1 Robustness.
Link:: https://www.sciencedirect.com/science/article/pii/S09280987183017517
No, please do not rinse the Membrane before use.
Yes, contact with water causes the membrane to bulge. As a result, the handling of the membrane can be difficult.
The biomimetic membrane is resistive against a lot of substances.
Here you can find the so far tested/ published substance:
[1] H. A. Bibi et al. (2015): Permeapad™ for investigation of passive drug permeability: The effect of surfactants, co-solvents and simulated intestinal fluids (FaSSIF and FeSSIF). International Journal of Pharmaceutics 493: 192-197
https://www.sciencedirect.com/science/article/pii/S0378517315300478
[2] M. di Cagno et al. (2015): New biomimetic barrier Permeapad™ for efficient investigation of passive permeability of drugs. European Journal of Pharmaceutical Sciences 73: 29-34
https://www.sciencedirect.com/science/article/pii/S0928098715001190
[3] H. A. Bibi et al. (2016): Use of Permeapad® for prediction of buccal absorption: A comparison to in vitro, ex vivo and in vivo method. European Journal of Pharmaceutical Sciences 93: 399-404
https://www.sciencedirect.com/science/article/pii/S092809871630330X
[4] H. A. Bibi et al. (2017): Simultaneous lipolysis/permeation in vitro model, for the estimation of bioavailability of lipid based drug delivery systems. European Journal of Pharmaceutics and Biopharmaceutics 117: 300-307.
https://www.sciencedirect.com/science/article/abs/pii/S0939641117301534
Yes, both products are disposable.
a) The term solubility has to differentiate between true (molecular) solubility and apparent solubility.
By strictly physical definition solubility is the solubility of a substance in a given pure solvent. For NCEs, this depends not only on the temperature but also on the pH or pka (in the case of dissociating substances) and thus also on the salt form (hydrochloride vs base) and on the crystal modification (solid state).
By the term “apparent solubility” more commonly used in the pharmaceutical field is meant both the above-mentioned true solubility plus all states of the substance which also contribute to the solubility, such as complexes (cyclodextrin) or micelles (surfactants). Since these states are u.a. can be influenced by formulation components, the apparent solubility is also often determined for formulations.
b) With the term dissolution rate, the pharmacist usually means the dissolution / release rate from a formulation. But one can also determine the dissolution rate of the pure NCE, which mostly depends strongly on the particle size. To eliminate the influence of the particle size, one can determine the “intrinsic dissolution rate” of the pure NCE.
We specify a storage period of 1 year, a use after longer-term storage should be easily possible.
You recognize a damaged barrier because of:
– cracks or air pockets between the two membranes
– the membranes are shifted against each other
It can be used for example in a Franz cell or a side-by-side diffusion cell. You can also create alternative structures.
Storage of PermeaPad<sup>®</sup> products:
– Store dry and dark at 25 ° C
– Protect from extreme temperatures
– Protect from dust and sunshine
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